Journal: Molecular imaging and biology

Article Title: 2 nd Window NIR Imaging of Radiation Injury Mitigation Provided by Reduced Notch-Dll4 Expression on Vasculature

doi: 10.1007/s11307-023-01840-7

Figure Lengend Snippet: Study design and number of animals for each experiment

Article Snippet: About ten million lung cells per animal were used for CD31 + enrichment using the CD31 Microbeads kit (cat# 130–109–680, Miltenyi) as per the manufacturer’s instructions.

Techniques: In Vivo, Imaging, Ex Vivo, Expressing, Immunostaining

Journal: Molecular imaging and biology

Article Title: 2 nd Window NIR Imaging of Radiation Injury Mitigation Provided by Reduced Notch-Dll4 Expression on Vasculature

doi: 10.1007/s11307-023-01840-7

Figure Lengend Snippet: SS.BN3 rats have reduced Dll4 expression and reduced susceptibility to late radiation-induced morbidity. a Consomic map illustrating the SS.BN3 rat is generated by the substitution of chromosome 3 from the Brown Norway rat into the Dahl-SS genetic background. b mRNA expression of Dll4 from CD31+ endothelial cells in non-irradiated SS (Dll4-high) and consomic SS.BN3 (Dll4-low) rats (n = 4 (SS) or 5 (SS.BN3) rats per group). c Survival following 13 Gy partial body irradiation (PBI) in adult SS (n = 13) and SS.BN3 rats (n = 19). P value for log-rank analysis: P = 0.0002 for SS vs SS.BN3 following 13 Gy PBI. d Change in body weight relative to pre-irradiation body weight for the rats represented in the survival curve (**P = 0.0066, ****P < 0.0001, two-way ANOVA Sidak’s multiple testing correction). Note the increased drop in body weight at 90 and 120 days in SS as compared to SS.BN3 rats, that occurs during pneumonitis and nephropathy, respectively

Article Snippet: About ten million lung cells per animal were used for CD31 + enrichment using the CD31 Microbeads kit (cat# 130–109–680, Miltenyi) as per the manufacturer’s instructions.

Techniques: Expressing, Generated, Irradiation

Journal: Molecular imaging and biology

Article Title: 2 nd Window NIR Imaging of Radiation Injury Mitigation Provided by Reduced Notch-Dll4 Expression on Vasculature

doi: 10.1007/s11307-023-01840-7

Figure Lengend Snippet: SS.BN3 (Dll4-low) rats have reduced radiation-induced loss of CD31+ endothelial cells. a Left, representative FACS contour plots showing the percentage of CD45−CD31+ lung ECs at day 70 following 13 Gy partial body irradiation in SS (Dll4-high) and SS.BN3 (Dll4-low) rats. Right, quantification of percent CD45−CD31+ cells (n = 9 rats per group). b Left, representative FACS contour plots showing the percentage of CD45−CD31+ kidney ECs at day 70 following 13 Gy partial body irradiation in SS (Dll4-high, n = 4) and SS.BN3 (Dll4-low, n = 5) rats. Right, quantification of percent CD45−CD31+ cells

Article Snippet: About ten million lung cells per animal were used for CD31 + enrichment using the CD31 Microbeads kit (cat# 130–109–680, Miltenyi) as per the manufacturer’s instructions.

Techniques: Irradiation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 2. mRNA expression levels of VEGF and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Expressing, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 3. Immunohistochemical analysis of VEGF expression in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Immunohistochemistry staining of VEGF in the articular cartilage of the medial tibial plateau (magnification, x400, scale bar=100 µm). (B) Quantification of VEGF positive cells, based on the results of immunohistochemistry staining. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Immunohistochemical staining, Expressing, Immunohistochemistry, Staining, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 5. ELISA analysis of serum VEGF concentration of mice among the Sham, Dmm and Dmm+Th groups (n=8 in each group). The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation